Journal: Frontiers in Pharmacology

Article Title: Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology

doi: 10.3389/fphar.2021.609702

Figure Lengend Snippet: Experimental validation of key signaling pathways and mitochondrial key targets in vivo . Dried ginger-aconite decoction(DAD) activates the PI3k/Akt/GSK-3β signaling pathway and inhibits the expression of Cyt-C and CASP9. Data were presented as mean standard deviation (SD) of three independent experiments. # p < 0.05, ## p < 0.01 vs. sham group. * p < 0.05, ** p < 0.01 vs. MI/RI group.

Article Snippet: Cyt-C, β -action, GADPH, Casp9, PI3K, AKT, Bax, Bcl-2 and phosphorylated( P )-AKT, GSK3β were also purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Protein-Protein interactions, In Vivo, Expressing, Standard Deviation

Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown

Journal: Experimental and Therapeutic Medicine

Article Title: Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation

doi: 10.3892/etm.2021.10070

Figure Lengend Snippet: GA affects the apoptosis and proliferation of HCT116 and HT29 colon cancer cells. (A) Plate clone formation assay was used to examine cell proliferation. (B) Flow cytometry was used to examine apoptosis. (C) Western blotting was used to analyze the expression of proliferation-related protein Ki-67 and apoptosis-related proteins, cleaved caspase-3, pro-caspase-3, cleaved caspase-9 and pro-caspase-9 (n=3). * P<0.05 and ** P<0.01 vs. the control group; # P<0.05 and ## P<0.01 vs. the GA-L group; @ P<0.05 and @@ P<0.01 vs. the GA-M group. GA, gallic acid; L, low dose; M, medium dose; H, high dose; 5-FU, 5-fluorouracil.

Article Snippet: In total, 50 µg protein samples were separated by 10% SDS-PAGE and then transferred to PVDF membranes for 2 h. After blocking with 5% non-fat dry milk at room temperature for 2 h, primary antibodies against cleaved caspase-9 (cat. no. orb227889; 1:1,000; Biorbyt Ltd.), pro-caspase-9 (ab138412; 1:800), cleaved caspase-3 (cat. no. ab49822; 1:500), pro-caspase-3 (ab32150; 1:1,000; all from Abcam), Ki-67 (cat. no. orb475270; 1:200; Biorbyt Ltd.), rabbit anti-human phosphorylated (p)-EGFR (cat. no. ab32578; 1:1,000), EGFR (cat. no. ab52894; 1:1,000), p-AKT (cat. no. ab38449; 1:1,000), AKT (cat. no. ab8805; 1:500), p-SRC (cat. no. ab32078; 1:5,000), SRC (cat. no. ab109381; 1:10,000), p-STAT3 (cat. no. ab76315; 1:2,000), STAT3 (cat. no. ab68153; 1:1,000) and β-actin (cat. no. ab8227; 1:1,000; all from Abcam) were added to the membranes and incubated overnight at 4˚C.

Techniques: Tube Formation Assay, Flow Cytometry, Western Blot, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation

doi: 10.3892/etm.2021.10070

Figure Lengend Snippet: GA decreases SRC and EGFR phosphorylation to promote apoptosis and inhibit proliferation in HCT116 and HT29 cells. (A) Plate clone formation assay was used to examine cell proliferation. (B) Flow cytometry was used to examine apoptosis. (C) Western blotting was used to analyze the expression of proliferation-related protein Ki-67 and apoptosis-related proteins, cleaved caspase-3, pro-caspase-3, cleaved caspase-9 and pro-caspase-9. n=3. ** P<0.01 vs. the control group; %% P<0.01 vs. the GA-H group; $ P<0.05 and $$ P<0.01 vs. the PP2 group. GA-H, gallic acid-high dose.

Article Snippet: In total, 50 µg protein samples were separated by 10% SDS-PAGE and then transferred to PVDF membranes for 2 h. After blocking with 5% non-fat dry milk at room temperature for 2 h, primary antibodies against cleaved caspase-9 (cat. no. orb227889; 1:1,000; Biorbyt Ltd.), pro-caspase-9 (ab138412; 1:800), cleaved caspase-3 (cat. no. ab49822; 1:500), pro-caspase-3 (ab32150; 1:1,000; all from Abcam), Ki-67 (cat. no. orb475270; 1:200; Biorbyt Ltd.), rabbit anti-human phosphorylated (p)-EGFR (cat. no. ab32578; 1:1,000), EGFR (cat. no. ab52894; 1:1,000), p-AKT (cat. no. ab38449; 1:1,000), AKT (cat. no. ab8805; 1:500), p-SRC (cat. no. ab32078; 1:5,000), SRC (cat. no. ab109381; 1:10,000), p-STAT3 (cat. no. ab76315; 1:2,000), STAT3 (cat. no. ab68153; 1:1,000) and β-actin (cat. no. ab8227; 1:1,000; all from Abcam) were added to the membranes and incubated overnight at 4˚C.

Techniques: Tube Formation Assay, Flow Cytometry, Western Blot, Expressing